A549-CAS9 cell line

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A549-CAS9 Order

Catalog#

YC-C016-Cas9-H

Size

1*10^6

Transfection Culture Medium

Transfection Culture Medium(A549)

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

A549-CAS9

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90% F12K+10% FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the A549 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a A549-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
A549-CAS9	Cas9	15.90389538	0.20190239
A549-CAS9	β-actin	15.70199299	0.20190239
A549	Cas9	35.62637711	20.59250927
A549	β-actin	15.03386784	20.59250927

Cutting Efficiency Validation

A549-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the A549-CAS9 stable cell pool which is electroporated by ANGPTL4 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:A549			STR Info (Cell bank) Cell Line:A-549
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	11		11	11
D13S317	11	11		11	11
D7S820	8	11		8	11
D16S539	11	12		11	12
VWA	14	14		14	14
TH01	8	9.3		8	9.3
AMEL	X	Y		X	Y
TPOX	8	11		8	11
CSF1PO	10	12		10	12
D12S391	18	18
FGA	23	23
D2S1338	24	24
D21S11	29	29
D18S51	14	17
D8S1179	13	14
D3S1358	16	16
D6S1043	11	13
PENTAE	7	11
D19S433	13	13
PENTAD	9	9
D1S1656	17	18.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

A549-CAS9

Catalog#

YC-C016-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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A549-CAS9 cell line

Ubigene